Purdue Cryo-EM Facility » software. DM4, mrcs, and essentially all formats used in cryo-EM fields by using EMAN2 IO functions Automatically convert to native endian of current computer regardless of starting endianness i.e. from big-endian used by DM4 images to little-endian used by Intel CPUs. 10/12/2019 · The Holiday Inn City Centre in Downtown Lafayette, IN is the primary hotel location for our symposium. You can call and request a room reservation at a discounted rate by mentioning the 2018 Purdue Cryo-EM Conference before September 24th — rooms are limited and reservations after this period will be charged standard rates. Purdue Cryo-EM Facility » Equipment Equipment. TEM Titan Krios. post-GIF K3 Direct Electron Detector. Purdue University Biological Sciences 915 W. State Street West Lafayette, IN 47907-2054 Main Office:765 494-4408 Business Office: 765 494-4764.
The Purdue CryoVR Project. Cryo-EM has received global recognition for its capacity to resolve high-resolution structures of macromolecules, and deservingly, was the topic of the 2017 Nobel Prize in Chemistry. This recent spike in attention to cryo-EM has developed a major need for training tools for incoming scientists. The Purdue CryoVR Project Cryo-EM has received global recognition for its capacity to resolve high-resolution structures of macromolecules, and deservingly, was the topic of the 2017 Nobel Prize in Chemistry. This recent spike in attention to cryo-EM has developed a. News. As mentioned before by other cryo-EM software developers, pixel box size does significantly affect computational time. Below is a table indicating the optimal box sizes for image processing. The best sizes to use are even numbers with a low prime factors such. In the Jiang Lab, we are interested in technology development and applications of cryo-electron microscopy cryo-EM imaging and 3-D reconstruction of viruses and macromolecular complexes, nanobiology, diseases, and drug discovery. Purdue Cryo-EM Facility » Contact Us Contact Us. Location: Suite 171, Hockmeyer Hall for Structural Biology, Purdue University. Email: Thomas Klose
Space is limited and we ask that guests first fill out a pre-registration form, provided below. Once pre-registration information is submitted and reviewed, the committee will send out invitations via e-mail the first week of September. Through this link, guests can pay the. Cryo-electron microscopy is now the most popular method for determining protein structures, which helps researchers develop drugs for different kinds of ailments. Over the last several decades, it has replaced X-ray crystallography because it can image proteins that can’t easily be formed into large crystals. The new technique was so. Jiang Lab Intranet. This wiki site is for internal use by Jiang lab members only. You must be authorized to view/edit the contents. To login, click on the "log in. We use cryo-EM to study the structures and functions of viruses, protein complexes, and nano-particles. We are also interested in the development of cryo-EM techniques including stablization of protein conformations, affinity grids, TEM alignment, data collection automation, and image processing/3D reconstruction algorithms. Purdue University is an equal access/equal opportunity university. If you have trouble accessing this page because of a disability. please contact the College of Science Webmaster.
|Purdue Cryo-EM Facility » People Prof. Wen Jiang. Scientific Director jiang12@ 765496-8436. Thomas Klose, Ph.D. Technical. Purdue University Biological Sciences 915 W. State Street West Lafayette, IN 47907-2054 Main Office:765 494-4408 Business Office: 765 494-4764.||06/11/2019 · Check out our campus tours during this time! Tour guides will be departing from Shively at 1:45pm. Posters will be setup at both ends of the venue. Presenters can setup anytime before 4:00pm on Wednesday and they can be left up until the end of the day.||07/11/2018 · Join us for our campus tour on Wednesday, November 7th, 2018! Take part in our graduate student-organized campus tour. Weather permitting, get a ride from the one and only Boilermaker Special. Look around our biophysics facilities and take a.||There are two options when considering using the Purdue Cryo-EM facility: 1 personnel training or 2 purchasing full service. If you only have one specific project involving cryo-EM, it will be cheaper to purchase a full service session, which will yield results faster.|
Single particle cryo-EM is revolutionizing structural biology. Many structures of viruses and protein complexes have been determined to 2-4 Å resolutions. While stable structures that can be expressed/purified in large quantities can be solved routinely, the dynamic compositions and. jspr /ˈjaspər/ is a collection of the programs for single particle cryo-EM image processing and 3-D reconstruction that are developed in Wen Jiang group in the Department of Biological Sciences, Purdue. Welcome to the Purdue Cryo-EM Facility Youtube Channel. Faculty and students in the group participate in a large number of interdisciplinary activities and shared resources including the Purdue Cryo-EM Facility, Purdue Center for Cancer Research, Center for Basic and Applied Membrane Sciences, Energy Center, the Purdue Institute of Inflammation, Immunology, and Infectious Disease PI 4 D, the.
The Purdue Cryo EM Facility became a fee-for-service recharge facility in 2011. Since that time we have provided a range of cryo electron microscopy services for Purdue and non- Purdue researchers. We specialize in high resolution transmission electron microscopy of frozen particulate samples, a.k.a Cryo EM. Our lab is on the first floor of the building adjacent to the Purdue Cryo-EM Facility. Hockmeyer houses the Titan Krios equipped with a K3 detector, a Talos F200C with a K2 camera along with other cryo-EM sample preparation instruments and computers for image processing and 3D reconstructions.
The Life Science Microscopy Facility LSMF provides Purdue researchers access to state-of-the-art electron microscopy instrumenta tion and to provide service, consultation and training to assist scientists in achieving their research objectives. 22/04/2016 · We also acknowledge the use of the Purdue cryo-EM facility. We acknowledge E. Frye for help with sequence alignments. We also acknowledge the use of the Purdue cryo-EM facility. We are grateful to W. Jiang for his jspr program, which was used to. An Approach to Perform Cryo-EM Analysis of Glutaraldehyde Fixed BSL-3 Viruses in BSL-1/2 Conditions. Amar D Parvate, Purdue University. Abstract. Scientific investigations of biosafety level 3 BSL-3 agents require special containment facilities and highly trained staff. Postdoc position in cryo-electron microscopy at Purdue University, USA. Description: A postdoc position is available in the Jiang laboratory at the Markey Center of Structural Biology and Department of Biological Sciences, Purdue University, West Lafayette, Indiana, USA. 18/10/2019 · Cryo-EM structure of immature Zika virus. Surface view a and c and cross-section b and d of mature 8 and immature ZIKV colored radially. Black arrows indicate the density between the inner RNA core and the viral membrane double-ended arrow shows the inside and outside layers of the membrane in immature ZIKV.
The Jiang Lab Cryo-EM Lab, Purdue University. Research; Publications; Software; Team; Blog; Log In; Links. Research; Publications; Software; Team; Blog; Log In; Links.
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